Confotec® NR500
Description
Specification
Theory & Capabilities

General characteristics of the confocal microscope Confotec® NR500

Spatial resolution

LaserObjective lens: magnification and numerical aperture NAXY spatial resolutionZ spatial resolution
488 nm100X
NA = 0.95
295 nm450 nm
633 nm100X
NA = 0.95
395 nm590 nm
785 nm100X
NA = 0.95
560 nm750 nm
  • Laser 488 nm:
    objective lens magnification = 100X
    numerical aperture NA = 0.95
    XY spatial resolution = 295 nm
    Z spatial resolution = 450 nm
  • Laser 633 nm:
    objective lens magnification = 100X
    numerical aperture NA = 0.95
    XY spatial resolution = 395 nm
    Z spatial resolution = 590 nm
  • Laser 785 nm:
    objective lens magnification = 100X
    numerical aperture NA = 0.95
    XY spatial resolution = 560 nm
    Z spatial resolution = 750 nm

Spectral range for Raman spectra detection:30 cm-1 ~ 6000 cm-1
(depends on the wavelength of the excitation laser)
Spectral resolution:0.25 cm-1 (75 l/mm Echelle grating)
Sensitivity:peak of the 4th order of the Raman spectrum of Si is detected within 1 min
Modes of scanning:1. Fast mapping: scanning of a laser beam along the surface of a sample with XY galvanoscanner.
2. Shifting a sample with XY motorized scanning stage near a fixed laser beam.
3. Combined mode for fast obtaining panoramic images with high spatial resolution: XY scanner (Fast mapping) + motorized microscope stage.
Maximum scanning field size in the “Fast mapping” mode:XY 150 х 150 μm (with objective lens 100х)
Period of registration for one frame 150 х 150 μм in the “Fast mapping” mode:3 s. / 1001 х 1001 pixels
PC control:fully automated

General characteristics

  • Spectral range for Raman spectra detection: 30 1/cm ~ 6000 1/cm (depends on the wavelength of the excitation laser)
  • Spectral resolution: 0.25 1/cm (75 l/mm Echelle grating)
  • Sensitivity: peak of the 4th order of the Raman spectrum of Si is detected within 1 min
  • Modes of scanning: 1. Fast mapping: scanning of a laser beam along the surface of a sample with XY galvanoscanner. 2. Shifting a sample with XY motorized scanning stage near a fixed laser beam. 3. Combined mode for fast obtaining panoramic images with high spatial resolution: XY scanner (Fast mapping) + motorized microscope stage.
  • Maximum scanning field size in the “Fast mapping” mode: XY 150 х 150 μm (with objective lens 100х)
  • Period of registration for one frame 150 х 150 μм in the “Fast mapping” mode: 3 s. / 1001 х 1001 pixels
  • PC control: fully automated

Optical microscope

Type, model (other types of inverted or upright microscopes available):inverted Nikon Ti-S and upright Nikon Ni-U
Motorized scanning stage:

  • travel range
  • accuracy (1 mm of shift)
  • XY repeatability
  • minimal step
automated

  • 114 х 75 mm
  • 0.06 μm
  • ± 1 μm
  • 0.02 μm
Micro objective lenses:100х NA-0.95
40х NA-0.75
20х NA-0.50 and other
Z-scanner:

  • objective translation range
  • objective translation step
  • repeatability
piezo scanner

  • 80 μm
  • 50 nm
  • < 6 nm
High-resolution digital video camera:

  • sensor
  • ADC
digital color CCD camera

  • 1/2″, 2048 x 1536 pixels
  • 10 bit, speed 12 frames/s
Laser radiation delivery:three-position turret
  • Type, model: inverted Nikon Ti-S and upright Nikon Ni-U. (Other types of inverted or upright microscopes available.)
  • Motorized scanning automated stage: travel range 114 х 75 mm, accuracy (1 mm of shift) 0.06 μm, XY repeatability ± 1 μm, minimal step 0.02 μm.
  • Micro objective lenses: 100х NA-0.95, 40х NA-0.75, 20х NA-0.50 and other.
  • Z-scanner = piezo scanner: objective translation range 80 μm, objective translation step 50 nm, repeatability < 6 nm.

  • High-resolution digital video camera = digital color CCD camera: sensor = 1/2″, 2048 x 1536 pixels; ADC = 10 bit, speed 12 frames/s.
  • Laser radiation delivery: three-position turret.

Optical-mechanical unit (OMU)

Optimized optics for spectral range:325 – 1050 nm (UV-VIS-NIR)
400 – 1100 nm (VIS-NIR)
Laser radiation delivery:triple- and quintuple input port
Laser beam attenuator:automated unit with VND filter, 0-3D
Polarizer (excitation channel) and analyzer (detection channel):Glan-Taylor prism (automated unit)
Laser beam expander:automated vario telescope, magnification factor 1.0 – 4.0x
Half-wave (λ/2) plate positioner:automated three- / five-position
Raman filter positioner:automated three- / five-position
Interference filter positioner:automated six-position
Pre-pinhole objective lens positioner:automated three-coordinated (X, Y, Z)
  • Optimized optics for spectral range: 325 — 1050 nm (UV-VIS-NIR); 400 — 1100 nm (VIS-NIR).
  • Laser radiation delivery: triple- and quintuple input port.
  • Laser beam attenuator: automated unit with VND filter, 0-3D.
  • Polarizer (excitation channel) and analyzer (detection channel): Glan-Taylor prism (automated unit).
  • Laser beam expander: automated vario telescope, magnification factor 1.0 – 4.0x.
  • Half-wave (λ/2) plate positioner: automated three- / five-position.
  • Raman filter positioner: automated three- / five-position.
  • Interference filter positioner: automated six-position.
  • Pre-pinhole objective lens positioner: automated three-coordinated (X, Y, Z).

Imaging monochromator-spectrograph MS5004i with astigmatism compensation

Optical configuration:vertical
Focal length:520 mm
Magnification:1.0 vertical, 1.0 horizontal
Vertical spatial resolution:< 20 μm
Flat field size:28 х 5 mm
Stray light:1 х 10-5 (20 nm from laser line 633 nm)
Diffraction grating unit:automated four-position turret
Spectral resolution:
(wavelength 500 nm, CCD pixel 12 x 12 μm)
0.25 cm-1 (Echelle grating 75 l/mm)
0.9 cm-1 (grating 1800 l/mm)
Entrance spectral slit:automated confocal pinhole,
smoothly regulated from 0 to 1.5 mm
Output spectral slit:automated, smoothly regulated from 0 to 2 mm
Ports:1 input, 2 output
Output ports switching:automated output mirror
  • Optical configuration: vertical.
  • Focal length: 520 mm.
  • Magnification: 1.0 vertical, 1.0 horizontal.
  • Vertical spatial resolution: < 20 μm.

  • Flat field size: 28 х 5 mm.
  • Stray light: 1 х 10-5 (20 nm from laser line 633 nm).
  • Diffraction grating unit: automated four-position turret.
  • Spectral resolution (wavelength 500 nm, CCD pixel 12 x 12 μm): 0.25 1/cm (Echelle grating 75 l/mm); 0.9 1/cm (grating 1800 l/mm).

  • Entrance spectral slit: automated confocal pinhole, smoothly regulated from 0 to 1.5 mm.
  • Output spectral slit: automated, smoothly regulated from 0 to 2 mm.
  • Ports: 1 input, 2 output.
  • Output ports switching: automated output mirror.

Spectral camera for spectrograph

Type:digital CCD camera
Sensor type:back-thinned CCD sensor
2048 х 122 pixels
Pixel size:12 x 12 μm
Pixel area size:24.576 x 1.464 mm (width x height)
Spectral response range:from 200 to 1100 nm
Cooling with temperature stabilization:two-stage Peltier element, min –45 °С
ADC:16 bit
Sensitivity:1 photon for 1 ADC reading (at the max. sensitivity of 650 nm)
Dynamic range:not less than 10 000
  • Type: digital CCD camera.
  • Sensor type: bback-thinned CCD sensor 2048 х 122 pixels.
  • Pixel size: 12 x 12 μm.
  • Pixel area size: 24.576 x 1.464 mm (width x height).
  • Spectral response range: from 200 to 1100 nm.
  • Cooling with temperature stabilization: two-stage Peltier element, min –45 °С.
  • ADC: 16 bit.
  • Sensitivity: 1 photon for 1 ADC reading (at the max. sensitivity of 650 nm).
  • Dynamic range: not less than 10 000.

Fast scanning unit X, Y

Scanners:galvanometer scanners with X, Y mirrors
Scanning modes:raster high-speed and start-stop
Positioning accuracy:30 nm
Scanning area:150 μm х 150 μm (with 100Х objective lens)
Scanning speed:3 s/frame 1001 х 1001 pixels
  • Scanners: galvanometer scanners with X, Y mirrors.
  • Scanning modes: raster high-speed and start-stop.
  • Positioning accuracy: 30 nm.
  • Scanning area: 150 μm х 150 μm (with 100Х objective lens).
  • Scanning speed: 3 s/frame 1001 х 1001 pixels.

Unit of confocal laser microscope

Pre-pinhole objective lens positioner:automated three-coordinate (X, Y, Z)
Confocal pinhole:automated confocal pinhole,
smoothly regulated from 0 to 1.5 mm
Detector:Hamamatsu Photosensor module H6780-01
  • Pre-pinhole objective lens positioner: automated three-coordinate (X, Y, Z).
  • Confocal pinhole: automated confocal pinhole, smoothly regulated from 0 to 1.5 mm.
  • Detector: Hamamatsu Photosensor module H6780-01.

Lasers

Up to 5 lasers can be used simultaneously

Type of laser:Wavelength, nmOutput power, mW
He-Cd Laser (Single Mode (TEM00) He-Cd):32515, 30, 40, 50
Diode-pumped Laser (DPSS):47325, 50
Diode-pumped Laser (DPSS):53225, 50
He-Ne Laser:63310
Diode-pumped laser (DPSS):78580
Using of other types of lasers with wavelength from 350 to 850 nm is possible
  • He-Cd Laser (Single Mode (TEM00) He-Cd): wavelength 325 nm, output power 15, 30, 40 and 50 mW.
  • Diode-pumped Laser (DPSS): wavelength 473 nm, output power 25 and 50 mW.
  • Diode-pumped Laser (DPSS): wavelength 532 nm, output power 25 and 50 mW.
  • He-Ne Laser: wavelength 633 nm, output power 10 mW.
  • Diode-pumped laser (DPSS): wavelength 785 nm, output power 80 mW.
  • Using of other types of lasers with wavelength from 350 to 850 nm is possible.