Confotec® MR350 / MR520 / MR750
Description & Applications
Specification

General characteristics of the system of Confotec® MR series microscope

Spatial resolution

LaserObjective: magnification and NA LateralResolution (XY) AxialResolution (Z)
473 nm100x, NA = 0.95390 nm550 nm
532 nm100x, NA = 0.95440 nm620 nm
633 nm100x, NA = 0.95520 nm730 nm
785 nm100x, NA = 0.95650 nm910 nm
  • Laser 473 nm:
    objective magnification = 100x
    objective NA Lateral = 0.95
    resolution (XY) Axial = 390 nm
    resolution (Z) = 550 nm
  • Laser 550 nm:
    objective magnification = 100x
    objective NA Lateral = 0.95
    resolution (XY) Axial = 440 nm
    resolution (Z) = 620 nm
  • Laser 633 nm:
    objective magnification = 100x
    objective NA Lateral = 0.95
    resolution (XY) Axial = 520 nm
    resolution (Z) = 730 nm
  • Laser 785 nm:
    objective magnification = 100x
    objective NA Lateral = 0.95
    resolution (XY) Axial = 650 nm
    resolution (Z) = 910 nm

Spectral parameters

Spectral range for Raman spectra detection:60 сm-1 ~ 12000 сm-1
(depends on the laser excitation wavelength)
Spectral resolution:not less than 0.145 cm-1
(Echelle grating, 75 l/mm , model Confotec® MR520)
  • Spectral range for Raman spectra detection: 60 1/сm ~ 12000 1/сm (depends on the laser excitation wavelength).
  • Spectral resolution: not less than 0.145 1/cm (Echelle grating, 75 l/mm , Confotec® MR520).

Scanning

Confotec® MR350-SRMSp
Confotec® MR520-SRMSp
Confotec® MR750-SRMSp
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.
  • High speed and high spatial resolution panoramic imaging (Raman and Rayleigh scattering) using high speed PMTs, XY galvano-mirrors and an automated microscope stage.
Confotec® MR350-SRSp
Confotec® MR520-SRSp
Confotec® MR750-SRSp
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.
Confotec® MR350-SRM
Confotec® MR520-SRM
Confotec® MR750-SRM
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.
  • High speed and high spatial resolution panoramic Rayleigh scattering imaging using a Reflected unit, XY galvano-mirrors and an automated microscope stage.
Confotec® MR350-SR
Confotec® MR520-SR
Confotec® MR750-SR
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.
Confotec® MR350-S
Confotec® MR520-S
Confotec® MR750-S
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • Confotec® MR350-SRMSp, MR520-SRMSp, MR750-SRMSp:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.

    • High speed and high spatial resolution panoramic imaging (Raman and Rayleigh scattering) using high speed PMTs, XY galvano-mirrors and an automated microscope stage.

  • Confotec® MR350-SRSp, MR520-SRSp, MR750-SRSp:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.

  • Confotec® MR350-SRM, MR520-SRM, MR750-SRM:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.

    • High speed and high spatial resolution panoramic Rayleigh scattering imaging using a Reflected unit, XY galvano-mirrors and an automated microscope stage.

  • Confotec® MR350-SR, MR520-SR, MR750-SR:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.

  • Confotec® MR350-S, MR520-S, MR750-S:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

Microscope

Type, model:Upright Nikon Ni-U
Objective lenses:100х NA-0.95, 40х NA-0.75, 20х NA-0.50, etc.
Eyepieces:10x (optional 12.5x, 15x)
Motorized stage:for SRMSp and SRM configurations
  • travel range XY
  • position repeatability
  • metric accuracy
  • minimal step
  • X=114 mm, Y=75 mm
  • < 0.5 μm
  • 0.06 μm / 1 mm
  • 0.02 μm
Manual focusing along Z:
  • travel range
  • focusing mechanism sensitivity
  • 10 mm
  • 0.1 mm/turn
Z-scanner:
  • objective lens travel range
  • minimal step
  • repeatability
  • 80 μm
  • 50 nm
  • < 6 nm
High-resolution digital camera:with Peltier cooling (the temperature of 20 °C below the ambient temperature)
  • type
  • sensor
  • pixel size
  • ADC
  • cooling
  • digital color CCD camera
  • 4/3″, 2048 x 2048 pixels
  • 7.4 x 7.4 μm
  • 14 bit, 16 frames/sec
  • Peltier cooling
High-resolution digital camera:without cooling
  • type
  • sensor
  • pixel size
  • ADC
  • digital color CCD camera
  • 1/2″, 2048 x 1536 pixels
  • 3.2 x 3.2 μm
  • 10 bit, 12 frames/sec
Laser light input:three-position automatic turret
  • Type, model: Upright Nikon Ni-U.
  • Objective lenses: 100х NA-0.95, 40х NA-0.75, 20х NA-0.50, etc.
  • Eyepieces: 10x (optional 12.5x, 15x).
  • Motorized stage (for SRMSp and SRM configurations): travel range XY – X=114 mm, Y=75 mm; position repeatability < 0.5 μm; metric accuracy 0.06 μm / 1 mm; minimal step 0.02 μm.

  • Manual focusing along Z: travel range = 10 mm; focusing mechanism sensitivity = 0.1 mm/turn.
  • Z-scanner: objective lens travel range 80 μm; minimal step 50 nm; repeatability < 6 nm.

  • High-resolution digital camera with Peltier cooling (the temperature of 20 °C below the ambient temperature): type = digital color CCD camera; sensor = 4/3″, 2048 x 2048 pixels; pixel size = 7.4 x 7.4 μm; ADC = 14 bit, 16 frames/sec; cooling = Peltier cooling.
  • High-resolution digital camera without cooling: type = digital color CCD camera; sensor = 1/2″, 2048 x 1536 pixels; pixel size = 3.2 x 3.2 μm; ADC = 10 bit, 12 frames/sec.
  • Laser light input: three-position automatic turret.

Raman optical unit

Optimized optics for the spectral range:VIS and NIR (400-1100 nm)
Laser radiation delivery:built-in lasers – direct input;
external lasers: 633 nm – fiber input,
785 nm – direct input
Beam expander:magnification factor
2х – 10х
Edge filters unit:single position unit with two Edge filters, manual change
Confocal pinhole:optical fiber (Ø 50 μm)
Laser beam attenuator:continuously Variable Neutral Density Filter (VND),
optical density range of 0 – 3
  • Optimized optics for the spectral range: VIS and NIR (400-1100 nm).
  • Laser radiation delivery: built-in lasers – direct input; external lasers: 633 nm – fiber input, 785 nm – direct input.
  • Beam expander: magnification factor 2х – 10х.
  • Edge filters unit: single position unit with two Edge filters, manual change.
  • Confocal pinhole: optical fiber (Ø 50 μm).
  • Laser beam attenuator: continuously Variable Neutral Density Filter (VND), optical density range of 0 – 3.

Imaging monochromator-spectrograph

Microscope modelConfotec® MR350Confotec® MR520Confotec® MR750
Monochromator-spectrograph modelMS3504iMS5204iMS7504i
Optical scheme:Czerny-TurnerCzerny-TurnerCzerny-Turner
Ports:1 input, 2 output1 input, 2 output1 input, 2 output
Focal length (output):350520750
F/number (entrance):1 / 3.81 / 5.41 / 8.9
Horizontal magnification:1.091.091.05
Vertical magnification:1.491.291.125
Vertical spatial resolution (astigmatism):< 15 μm< 15 μm< 15 μm
Reciprocal linear dispersion:*2.37 nm/mm*1.50 nm/mm*1.50 nm/mm
Spectral resolution:*0.07 nm**0.007 (0.25 cm-1) nm*0.02 nm
Wavelength accuracy:*±0.125 nm*±0.05 nm*±0.05 nm
Repeatability:*±0.03 nm*±0.008 nm*±0.006 nm
Grating mounting:automated 4-grating turretautomated 4-grating turretautomated 4-grating turret
Grating wavelength repeatability:*±0.03 nm*±0.008 nm*±0.006 nm
Grating vertical image repeatability:±50 μm±50 μm±50 μm
* With 1200 l/mm grating and 15 µm slit at 546 nm.
** With Echele grating (75 l/mm) and 15 µm slit at 546 nm, order 44.

Microscope Confotec® MR350
Monochromator-spectrograph MS3504i

  • Optical scheme: Czerny-Turner

  • Ports: 1 input, 2 output

  • Focal length (output): 350

  • F/number (entrance): 1 / 3.8

  • Horizontal magnification: 1.09

  • Vertical magnification: 1.49

  • Vertical spatial resolution (astigmatism): < 15 μm

  • Reciprocal linear dispersion: *2.37 nm/mm

  • Spectral resolution: *0.07 nm

  • Wavelength accuracy: *±0.125 nm
  • Repeatability: *±0.03 nm
  • Grating mounting: automated 4-grating turret
  • Grating wavelength repeatability: *±0.03 nm
  • Grating vertical image repeatability: ±50 μm

Microscope Confotec® MR520
Monochromator-spectrograph MS5204i

  • Optical scheme: Czerny-Turner

  • Ports: 1 input, 2 output

  • Focal length (output): 520
  • F/number (entrance): 1 / 5.4
  • Horizontal magnification: 1.09

  • Vertical magnification: 1.29
  • Vertical spatial resolution (astigmatism): < 15 μm

  • Reciprocal linear dispersion: *1.50 nm/mm
  • Spectral resolution: **0.007 (0.25 cm-1) nm

  • Wavelength accuracy: *±0.05 nm
  • Repeatability: *±0.008 nm
  • Grating mounting: automated 4-grating turret
  • Grating wavelength repeatability: *±0.008 nm
  • Grating vertical image repeatability: ±50 μm

Microscope Confotec® MR750
Monochromator-spectrograph MS7504i

  • Optical scheme: Czerny-Turner

  • Ports: 1 input, 2 output

  • Focal length (output): 750
  • F/number (entrance): 1 / 8.9
  • Horizontal magnification: 1.05
  • Vertical magnification: 1.125
  • Vertical spatial resolution (astigmatism): < 15 μm

  • Reciprocal linear dispersion: *1.50 nm/mm
  • Spectral resolution: *0.02 nm
  • Wavelength accuracy: *±0.05 nm
  • Repeatability: *±0.006 nm
  • Grating mounting: automated 4-grating turret
  • Grating wavelength repeatability: *±0.006 nm
  • Grating vertical image repeatability: ±50 μm

* With 1200 l/mm grating and 15 µm slit at 546 nm.
** With Echele grating (75 l/mm) and 15 µm slit at 546 nm, order 44.

Imaging monochromator-spectrograph MS 3504i

Grating, l/mm30060012001800
Spectral range for Raman detection, cm-1
Laser wave-length 473 nm0-120000-9400
Laser wave-length 532 nm0-97000-7000
Laser wave-length 633 nm0-67000-4000
Laser wave-length 785 nm0-36000-1000
Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1
Laser wave-length 473 nm7000421023101550
Laser wave-length 532 nm5700340018401210
Laser wave-length 633 nm428024701300820
Laser wave-length 785 nm29301650830480
Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1
Laser wave-length 473 nm12.8506.4403.1852.050
Laser wave-length 532 nm10.1655.0902.5051.590
Laser wave-length 633 nm7.1903.5951.7451.070
Laser wave-length 785 nm4.6752.3301.1000.620

Spectral range for Raman detection, cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 0-12000, 0-12000, 0-12000 and 0-9400 cm-1

  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 0-9700, 0-9700, 0-9700 and 0-7000 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 0-6700, 0-6700, 0-6700 and 0-4000 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 0-3600, 0-3600, 0-3600 and 0-1000 cm-1

Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 7000, 4210, 2310 and 1550 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 5700, 3400, 1840 and 1210 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 4280, 2470, 1300 and 820 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 2930, 1650, 830 and 480 cm-1

Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 12.850, 6.440, 3.185 and 2.050 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 10.165, 5.090, 2.505 and 1.590 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 7.190, 3.595, 1.745 and 1.070 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 4.675, 2.330, 1.100 and 0.620 cm-1

Imaging monochromator-spectrograph MS 5204i

Grating, l/mm300600180075/Echelle
Spectral range for Raman detection, cm-1
Laser wave-length 473 nm0-120000-110000-12000
Laser wave-length 532 nm0-97000-87000-9700
Laser wave-length 633 nm0-67000-58000-6700
Laser wave-length 785 nm0-36000-27000-3600
Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1
Laser wave-length 473 nm690035001100275
Laser wave-length 532 nm55002760850255
Laser wave-length 633 nm39101950570210
Laser wave-length 785 nm25401260330160
Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1
Laser wave-length 473 nm6.1503.0800.9750.250
Laser wave-length 532 nm4.8652.4300.7550.230
Laser wave-length 633 nm3.4401.7150.5050.190
Laser wave-length 785 nm2.2351.1100.2950.145

Spectral range for Raman detection, cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 0-12000, 0-12000, 0-11000 and 0-12000 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 0-9700, 0-9700, 0-8700 and 0-9700 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 0-6700, 0-6700, 0-5800 and 0-6700 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 0-3600, 0-3600, 0-2700 and 0-3600 cm-1

Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 6900, 3500, 1100 and 275 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 5500, 2760, 850 and 255 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 3910, 1950, 570 and 210 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 2540, 1260, 330 and 160 cm-1

Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 6.150, 3.080, 0.975 and 0.250 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 4.865, 2.430, 0.755 and 0.230 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 3.440, 1.715, 0.505 and 0.190 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 2.235, 1.110, 0.295 and 0.145 cm-1

Imaging monochromator-spectrograph MS 7504i

Grating, l/mm30060012001800
Spectral range for Raman detection, cm-1
Laser wave-length 473 nm0-120000-9300
Laser wave-length 532 nm0-97000-6900
Laser wave-length 633 nm0-67000-3900
Laser wave-length 785 nm0-36000-900
Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1
Laser wave-length 473 nm391021301070680
Laser wave-length 532 nm31501690840520
Laser wave-length 633 nm22801200580340
Laser wave-length 785 nm1520780360185
Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1
Laser wave-length 473 nm3.7851.9150.9200.570
Laser wave-length 532 nm3.0601.5100.7150.435
Laser wave-length 633 nm2.1601.0600.4900.285
Laser wave-length 785 nm1.4000.6800.3050.160

Spectral range for Raman detection, cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 0-12000, 0-12000, 0-12000 and 0-9300 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 0-9700, 0-9700, 0-9700 and 0-6900 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 0-6700, 0-6700, 0-6700 and 0-3900 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 0-3600, 0-3600, 0-3600 and 0-900 cm-1

Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 3910, 2130, 1070 and 680 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 3150, 1690, 840 and 520 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 2280, 1200, 580 and 340 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 1520, 780, 360 and 185 cm-1

Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 3.785, 1.915, 0.920 and 0.570 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 3.060, 1.510, 0.715 and 0.435 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 2.160, 1.060, 0.490 and 0.285 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 1.400, 0.680, 0.305 and 0.160 cm-1

Digital spectral CCD camera

Sensor type:back-thinned CCD, 2048 x 122 pixels
Pixel size:12 x 12 μm
Active area size:24.576 x 1.464 mm (W x H)
Wavelength range:from 200 to 1100 nm
Cooling with temperature stabilization:two stage Peltier cooling, up to -45 °С (water cooling)
ADC:16 bit
Sensitivity:1 photon / 1 count ADC (at 650 nm)
Dynamic range:not less than 10 000
Synchronization:internal, external
Interface:Ethernet
  • Sensor type: back-thinned CCD, 2048 х 122 pixels.
  • Pixel size: 12 x 12 μm.
  • Active area size: 24.576 x 1.464 mm (W x H).
  • Wavelength range: from 200 to 1100 nm.

  • Cooling with temperature stabilization: two stage Peltier cooling, up to -45 °С (water cooling).
  • ADC: 16 bit.
  • Sensitivity: 1 photon / 1 count ADC (at 650 nm).

  • Dynamic range: not less than 10 000.
  • Synchronization: internal, external.
  • Interface: Ethernet.

PMT for spectral measurements

A Hamamatsu PMT unit (model H7844) with the built-in one-stage air cooled Peltier element is used for Raman spectra detection:

Spectral range:185 – 900 nm
Photocathode light sensitivity:450 μА/lm
Anode light sensitivity:> 5000 А/lm
Anode dark current
(in 3 minutes after start cooling):
< 0,05 nA
Max. cooling temperature (ΔТ):20 ºС
  • Spectral range: 185 — 900 nm
  • Photocathode light sensitivity: 450 μА/lm
  • Anode light sensitivity: > 5000А/lm
  • Anode dark current (in 3 minutes after start cooling): less than 0,05 nA
  • Max. cooling temperature (ΔТ): 20 ºС

Fast scanning unit X, Y

Scanners:galvanometer scanners with X, Y mirrors
Scanning modes:raster high-speed and start-stop
Positioning accuracy:< 30 nm
Scanning area:100 μm х 100 μm (with 100Х objective lens)
Scanning speed:3 s/frame 1001 х 1001 pixels
  • Scanners: galvanometer scanners with X, Y mirrors.
  • Scanning modes: raster high-speed and start-stop.
  • Positioning accuracy: < 30 nm.

  • Scanning area: 100 μm х 100 μm (with 100Х objective lens).
  • Scanning speed: 3 s/frame 1001 х 1001 pixels.

Confocal laser microscope unit

Pre-pinhole lens positioner:three-axis (X, Y, Z), manual control
Confocal pinhole:unregulated, Ø 30 μm
Detector:Hamamatsu Photosensor module H6780-01
  • Pre-pinhole lens positioner: three-axis (X, Y, Z), manual control.
  • Confocal pinhole: unregulated, Ø 30 μm.
  • Detector: Hamamatsu Photosensor module H6780-01.

Lasers: built-in and external

532 nm built-in laser

Type:ОЕМ Diode-pumped solid-state laser (DPSS)
Wavelength:532 nm
Output power:22±2 mW
Output beam diameter:1.1 ± 0.1 mm
Beam divergence:< 0,6±0.1 mrad
Spectral line width:0.00001 nm
Coherence length:> 50 m
Transverse mode:TEMoo, M2 < 1.2 (1.05 typ.)
Polarization:> 100:1, horizontal
Long term power stability:< 2% / 8 h.
Warm-up time:< 10 minutes
  • Type: ОЕМ Diode-pumped solid-state laser (DPSS)
  • Wavelength: 532 nm
  • Output power: 22±2 mW
  • Output beam diameter: 1.1 ± 0.1 mm
  • Beam divergence: < 0,6±0.1 mrad

  • Spectral line width: 0.00001 nm
  • Coherence length: > 50 m
  • Transverse mode: TEMoo, M2 < 1.2 (1.05 typ.)

  • Polarization: > 100:1, horizontal

  • Long term power stability: < 2% / 8 h.

  • Warm-up time: < 10 minutes

473 nm built-in laser

Type:ОЕМ Diode-pumped solid-state laser (DPSS)
Wavelength:473 nm
Output power:50 mW
Output beam diameter:0.7 ± 0.05 mm
Beam divergence:< 1.2 mrad
Spectral bandwidth:< 30 MHz
Coherence length:> 10 m
Transverse mode:TEMoo, M2 < 1.1
Polarization:> 100:1, vertical
Long term power stability:< 3% / 8 h.
Warm-up time:< 10 minutes
  • Type: ОЕМ Diode-pumped solid-state laser (DPSS)

  • Wavelength: 473 nm
  • Output power: 50 mW
  • Output beam diameter: 0.7 ± 0.05 mm
  • Beam divergence: < 1.2 mrad

  • Spectral bandwidth: < 30 MHz

  • Coherence length: > 10 m
  • Transverse mode: TEMoo, M2 < 1.1

  • Polarization: > 100:1, vertical
  • Long term power stability: < 3% / 8 h.

  • Warm-up time: < 10 minutes

633 nm external laser

Type:Helium-neon laser
Wavelength:632,8 nm
Output power:> 10 mW
Output beam diameter:0.65 mm
Beam divergence:< 1.24 mrad
Spectral bandwidth:341 MHz
Mode composition:> 90 % TEMoo
Polarization:500:1
Long term power stability:< 5% / 8 h.
Beam point stability:< 0.03 mrad/°C
Warm-up time:< 15 minutes
  • Type: Helium-neon laser
  • Wavelength: 632,8 nm
  • Output power: > 10 mW

  • Output beam diameter: 0.65 mm
  • Beam divergence: < 1.24 mrad
  • Spectral bandwidth: 341 MHz
  • Mode composition: > 90 % TEMoo

  • Polarization: 500:1
  • Long term power stability: < 5% / 8 h.
  • Beam point stability: < 0.03 mrad/°C

  • Warm-up time: < 15 minutes

785 nm external laser

Type:ОЕМ Diode-pumped solid-state laser (DPSS)
Wavelength:785 ± 0.2 nm
Output power:100 mW
Output beam diameter:1.5 mm
Beam divergence:< 1.0 mrad
Spectral bandwidth:< 1 MHz
Beam quality:M2 < 1.65
Polarization:vertical ±5°
Long term power stability:< 1% / 8 h.
Warm-up time:< 10 minutes
  • Type: ОЕМ Diode-pumped solid-state laser (DPSS)
  • Wavelength: 785 ± 0.2 nm
  • Output power: 100 mW
  • Output beam diameter: 1.5 mm
  • Beam divergence: < 1.0 mrad

  • Spectral bandwidth: < 1 MHz

  • Beam quality: M2 < 1.65

  • Polarization: vertical ±5°
  • Long term power stability: < 1% / 8 h.
  • Warm-up time: < 10 minutes

Microscope models

Microscope configuration modelsConfotec® MR350/520/750-SRMSpConfotec® MR350/520/750-SRMConfotec® MR350/520/750-SRSpConfotec® MR350/520/750-SRConfotec® MR350/520/750-S
Mapping3 D (X Y Z)3 D (X Y Z)3 D (X Y Z)3 D (X Y Z)3 D (X Y Z)
Scanning:XY galvano + motorized stage, Z piezoXY galvano, Z piezoXY galvano, Z piezoXY galvano, Z piezo
Fast scanning for confocal Raman imaging:YesYes
Fast scanning for confocal laser imaging:YesYesYesYes
Data:2D, 3D2D, 3D2D, 3D2D, 3D2D, 3D
MicroscopeUprightUprightUprightUprightUpright
Color video CCD:YesYesYesYesYes
Raman optical unit
Location:integrated with an optical microscope
Optical alignment:no need
Rayleigh rejection filters:two edge filters, manually replaced
Imaging monochromator-spectrograph
Focal length:350/520/750 mm
Exit ports:21211
Exit port selection:automated exit mirror
Number of gratings:44444
Grating mounting:automated 4-grating turret
Light input:one-channel optical fiber
Digital spectral CCD camera
Number of pixels:2048 x 122
Pixel size:12 x 12 µm
Cooling with temperature stabilization:two-stage Peltier cooling with temperature stabilization, up to -45°С (water cooling)
Excitation lasers (1 built-in and 1 external)
Wavelength of built-in laser:473 nm or 532 nm
Light input of built-in laser:direct
Wavelength of external laser:633 nm or 785 nm
Light input of external laser:633 nm – fiber, 785 nm – direct
Laser beam diameter control:zoom beam expander, manual
Laser power control:continuous VND (OD0-3), motorized
Confocal pinhole
Confocal Raman imaging:fiber Ø 50 μм
Confocal laser imaging:fixed Ø 30 μмfixed Ø 30 μм
Reflected Unit:YesYesYesYes
Built-in calibration lamp:Yes
Spectral PMT:YesYes
Motorized microscope stage:YesYes

Confotec® MR350/520/750-SRMSp

  • Mapping 3D (X Y Z): scanning = XY galvano + motorized stage, Z piezo; fast scanning for confocal Raman imaging; fast scanning for confocal laser imaging; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 2; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.
  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; confocal laser imaging = fixed Ø 30 μm; Reflected Unit; built-in calibration lamp; spectral PMT; motorized microscope stage.

Confotec® MR350/520/750-SRM

  • Mapping 3D (X Y Z): scanning = XY galvano + motorized stage, Z piezo; fast scanning for confocal laser imaging; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 1; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.
  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; confocal laser imaging = fixed Ø 30 μm; Reflected Unit; built-in calibration lamp; motorized microscope stage.

Confotec® MR350/520/750-SRSp

  • Mapping 3D (X Y Z): scanning = XY galvano, Z piezo; fast scanning for confocal Raman imaging; fast scanning for confocal laser imaging; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 2; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.

  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; confocal laser imaging = fixed Ø 30 μm; Reflected Unit; built-in calibration lamp; spectral PMT.

Confotec® MR350/520/750-SR

  • Mapping 3D (X Y Z): scanning = XY galvano, Z piezo; fast scanning for confocal laser imaging; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 1; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.
  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; confocal laser imaging = fixed Ø 30 μm; Reflected Unit; built-in calibration lamp.

Confotec® MR350/520/750-S

  • Mapping 3D (X Y Z): scanning = XY galvano, Z piezo; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 1; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.
  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; built-in calibration lamp.