General characteristics of the confocal microscope Confotec® NR500
Spatial resolution
| Laser | Objective lens: magnification and numerical aperture NA | XY spatial resolution | Z spatial resolution |
|---|---|---|---|
| 488 nm | 100X NA = 0.95 | 295 nm | 450 nm |
| 633 nm | 100X NA = 0.95 | 395 nm | 590 nm |
| 785 nm | 100X NA = 0.95 | 560 nm | 750 nm |
- Laser 488 nm:
objective lens magnification = 100X
numerical aperture NA = 0.95
XY spatial resolution = 295 nm
Z spatial resolution = 450 nm - Laser 633 nm:
objective lens magnification = 100X
numerical aperture NA = 0.95
XY spatial resolution = 395 nm
Z spatial resolution = 590 nm Laser 785 nm:
objective lens magnification = 100X
numerical aperture NA = 0.95
XY spatial resolution = 560 nm
Z spatial resolution = 750 nm
| Spectral range for Raman spectra detection: | 30 cm-1 ～ 6000 cm-1 (depends on the wavelength of the excitation laser) |
| Spectral resolution: | 0.25 cm-1 (75 l/mm Echelle grating) |
| Sensitivity: | peak of the 4th order of the Raman spectrum of Si is detected within 1 min |
| Modes of scanning: | 1. Fast mapping: scanning of a laser beam along the surface of a sample with XY galvanoscanner. 2. Shifting a sample with XY motorized scanning stage near a fixed laser beam. 3. Combined mode for fast obtaining panoramic images with high spatial resolution: XY scanner (Fast mapping) + motorized microscope stage. |
| Maximum scanning field size in the “Fast mapping” mode: | XY 150 х 150 μm (with objective lens 100х) |
| Period of registration for one frame 150 х 150 μм in the “Fast mapping” mode: | 3 s. / 1001 х 1001 pixels |
| PC control: | fully automated |
General characteristics
- Spectral range for Raman spectra detection: 30 1/cm ～ 6000 1/cm (depends on the wavelength of the excitation laser)
- Spectral resolution: 0.25 1/cm (75 l/mm Echelle grating)
- Sensitivity: peak of the 4th order of the Raman spectrum of Si is detected within 1 min
- Modes of scanning: 1. Fast mapping: scanning of a laser beam along the surface of a sample with XY galvanoscanner. 2. Shifting a sample with XY motorized scanning stage near a fixed laser beam. 3. Combined mode for fast obtaining panoramic images with high spatial resolution: XY scanner (Fast mapping) + motorized microscope stage.
- Maximum scanning field size in the “Fast mapping” mode: XY 150 х 150 μm (with objective lens 100х)
- Period of registration for one frame 150 х 150 μм in the “Fast mapping” mode: 3 s. / 1001 х 1001 pixels
- PC control: fully automated
Optical microscope
| Type, model (other types of inverted or upright microscopes available): | inverted Nikon Ti-S and upright Nikon Ni-U |
Motorized scanning stage:
| automated
|
| Micro objective lenses: | 100х NA-0.95 40х NA-0.75 20х NA-0.50 and other |
Z-scanner:
| piezo scanner
|
High-resolution digital video camera:
| digital color CCD camera
|
| Laser radiation delivery: | three-position turret |
- Type, model: inverted Nikon Ti-S and upright Nikon Ni-U. (Other types of inverted or upright microscopes available.)
- Motorized scanning automated stage: travel range 114 х 75 mm, accuracy (1 mm of shift) 0.06 μm, XY repeatability ± 1 μm, minimal step 0.02 μm.
- Micro objective lenses: 100х NA-0.95, 40х NA-0.75, 20х NA-0.50 and other.
Z-scanner = piezo scanner: objective translation range 80 μm, objective translation step 50 nm, repeatability < 6 nm.
- High-resolution digital video camera = digital color CCD camera: sensor = 1/2″, 2048 x 1536 pixels; ADC = 10 bit, speed 12 frames/s.
- Laser radiation delivery: three-position turret.
Optical-mechanical unit (OMU)
| Optimized optics for spectral range: | 325 – 1050 nm (UV-VIS-NIR) 400 – 1100 nm (VIS-NIR) |
| Laser radiation delivery: | triple- and quintuple input port |
| Laser beam attenuator: | automated unit with VND filter, 0-3D |
| Polarizer (excitation channel) and analyzer (detection channel): | Glan-Taylor prism (automated unit) |
| Laser beam expander: | automated vario telescope, magnification factor 1.0 – 4.0x |
| Half-wave (λ/2) plate positioner: | automated three- / five-position |
| Raman filter positioner: | automated three- / five-position |
| Interference filter positioner: | automated six-position |
| Pre-pinhole objective lens positioner: | automated three-coordinated (X, Y, Z) |
- Optimized optics for spectral range: 325 — 1050 nm (UV-VIS-NIR); 400 — 1100 nm (VIS-NIR).
- Laser radiation delivery: triple- and quintuple input port.
- Laser beam attenuator: automated unit with VND filter, 0-3D.
- Polarizer (excitation channel) and analyzer (detection channel): Glan-Taylor prism (automated unit).
- Laser beam expander: automated vario telescope, magnification factor 1.0 – 4.0x.
- Half-wave (λ/2) plate positioner: automated three- / five-position.
- Raman filter positioner: automated three- / five-position.
- Interference filter positioner: automated six-position.
- Pre-pinhole objective lens positioner: automated three-coordinated (X, Y, Z).
Imaging monochromator-spectrograph MS5004i with astigmatism compensation
| Optical configuration: | vertical |
| Focal length: | 522 mm |
| Magnification: | 1.0 vertical, 1.0 horizontal |
| Vertical spatial resolution: | < 20 μm |
| Flat field size: | 28 х 5 mm |
| Stray light: | 1 х 10-5 (20 nm from laser line 633 nm) |
| Diffraction grating unit: | automated four-position turret |
| Spectral resolution: (effective, FWHM) | 0.25 cm-1 (Echelle grating 75 l/mm, wavelength 500 nm) 0.7 cm-1 (grating 1800 l/mm, wavelength 532 nm)0.5 cm-1 (grating 1800 l/mm, wavelength 633 nm) |
| Entrance spectral slit: | automated confocal pinhole, smoothly regulated from 0 to 1.5 mm |
| Output spectral slit: | automated, smoothly regulated from 0 to 2 mm |
| Ports: | 1 input, 2 output |
| Output ports switching: | automated output mirror |
- Optical configuration: vertical.
Focal length: 522 mm.
- Magnification: 1.0 vertical, 1.0 horizontal.
Vertical spatial resolution: < 20 μm.
- Flat field size: 28 х 5 mm.
- Stray light: 1 х 10-5 (20 nm from laser line 633 nm).
- Diffraction grating unit: automated four-position turret.
Spectral resolution (effective, FWHM): 0.25 1/cm (Echelle grating 75 l/mm, wavelength 500 nm); 0.7 1/cm (grating 1800 l/mm, wavelength 532 nm); 0.5 1/cm (grating 1800 l/mm, wavelength 633 nm)
- Entrance spectral slit: automated confocal pinhole, smoothly regulated from 0 to 1.5 mm.
- Output spectral slit: automated, smoothly regulated from 0 to 2 mm.
- Ports: 1 input, 2 output.
- Output ports switching: automated output mirror.
Spectral camera for spectrograph
| Pixel size: | 12 x 12 μm |
| Pixel area size: | 24.576 x 1.464 mm (width x height) |
| Spectral response range: | from 200 to 1100 nm |
| Cooling with temperature stabilization: | two-stage Peltier element, min –45 °С |
| ADC: | 16 bit |
| Sensitivity: | 1 photon for 1 ADC reading (at the max. sensitivity of 650 nm) |
| Dynamic range: | not less than 10 000 |
| Type: | digital CCD camera. Using a camera with 1024*256 pixels, 126 nm, with BackThinned or Front-Illuminated sensor type, cooling to -100°C is possible. |
- Pixel size: 12 x 12 μm.
- Pixel area size: 24.576 x 1.464 mm (width x height).
- Spectral response range: from 200 to 1100 nm.
- Cooling with temperature stabilization: two-stage Peltier element, min –45 °С.
- ADC: 16 bit.
- Sensitivity: 1 photon for 1 ADC reading (at the max. sensitivity of 650 nm).
- Dynamic range: not less than 10 000.
Type: digital CCD camera. Using a camera with 1024*256 pixels, 126 nm, with BackThinned or Front-Illuminated sensor type, cooling to -100°C is possible.
Fast scanning unit X, Y
| Scanners: | galvanometer scanners with X, Y mirrors |
| Scanning modes: | raster high-speed and start-stop |
| Positioning accuracy: | 30 nm |
| Scanning area: | 150 μm х 150 μm (with 100Х objective lens) |
| Scanning speed: | 3 s/frame 1001 х 1001 pixels |
- Scanners: galvanometer scanners with X, Y mirrors.
- Scanning modes: raster high-speed and start-stop.
- Positioning accuracy: 30 nm.
- Scanning area: 150 μm х 150 μm (with 100Х objective lens).
- Scanning speed: 3 s/frame 1001 х 1001 pixels.
Unit of confocal laser microscope
| Pre-pinhole objective lens positioner: | automated three-coordinate (X, Y, Z) |
| Confocal pinhole: | automated confocal pinhole, smoothly regulated from 0 to 1.5 mm |
| Detector: | Hamamatsu Photosensor module H6780-01 |
- Pre-pinhole objective lens positioner: automated three-coordinate (X, Y, Z).
- Confocal pinhole: automated confocal pinhole, smoothly regulated from 0 to 1.5 mm.
- Detector: Hamamatsu Photosensor module H6780-01.
Lasers
Up to 5 lasers can be used simultaneously
| Type of laser: | Wavelength, nm | Output power, mW |
|---|---|---|
| He-Cd Laser (Single Mode (TEM00) He-Cd): | 325 | 15, 30, 40, 50 |
| Diode-pumped Laser (DPSS): | 473 | 25, 50 |
| Diode-pumped Laser (DPSS): | 532 | 25, 50 |
| He-Ne Laser: | 633 | 10 |
| Diode-pumped laser (DPSS): | 785 | 80 |
| Using of other types of lasers with wavelength from 350 to 850 nm is possible | ||
- He-Cd Laser (Single Mode (TEM00) He-Cd): wavelength 325 nm, output power 15, 30, 40 and 50 mW.
- Diode-pumped Laser (DPSS): wavelength 473 nm, output power 25 and 50 mW.
- Diode-pumped Laser (DPSS): wavelength 532 nm, output power 25 and 50 mW.
- He-Ne Laser: wavelength 633 nm, output power 10 mW.
- Diode-pumped laser (DPSS): wavelength 785 nm, output power 80 mW.
- Using of other types of lasers with wavelength from 350 to 850 nm is possible.