Confotec® MR350 / MR520 / MR750
Description & Applications
Specification

General characteristics of the system of Confotec® MR series microscope

Spatial resolution

Laser Objective: magnification and NA Lateral Resolution (XY) Axial Resolution (Z)
Fiber coupled config.(core diameter 100 um) Free space spectrometer coupling Fiber coupled config.(core diameter 100 um) Free space spectrometer coupling
473 nm 100х, NA = 0,95 390 nm 300 550 nm 450
532 nm 100х, NA = 0,95 440 nm 330 620 nm  520
633 nm 100х, NA = 0,95 520 nm 400 730 nm 600
785 nm 100х, NA = 0,95 650 nm 600 910 nm 750
  • Laser 473 nm:
    objective magnification = 100x
    objective NA Lateral = 0.95
    resolution (XY) Axial = 390 nm
    resolution (Z) = 550 nm
  • Laser 550 nm:
    objective magnification = 100x
    objective NA Lateral = 0.95
    resolution (XY) Axial = 440 nm
    resolution (Z) = 620 nm
  • Laser 633 nm:
    objective magnification = 100x
    objective NA Lateral = 0.95
    resolution (XY) Axial = 520 nm
    resolution (Z) = 730 nm
  • Laser 785 nm:
    objective magnification = 100x
    objective NA Lateral = 0.95
    resolution (XY) Axial = 650 nm
    resolution (Z) = 910 nm

Spectral parameters

Spectral range for Raman spectra detection: 60 сm-1 ～ 12000 сm-1
(depends on the laser excitation wavelength)
Spectral resolution: not less than 0.145 cm-1
(Echelle grating, 75 l/mm , model Confotec® MR520)
  • Spectral range for Raman spectra detection: 60 1/сm ～ 12000 1/сm (depends on the laser excitation wavelength).

  • Spectral resolution: not less than 0.145 1/cm (Echelle grating, 75 l/mm , Confotec® MR520).

Scanning

Confotec® MR350-SRMSp
Confotec® MR520-SRMSp
Confotec® MR750-SRMSp
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.
  • High speed and high spatial resolution panoramic imaging (Raman and Rayleigh scattering) using high speed PMTs, XY galvano-mirrors and an automated microscope stage.
Confotec® MR350-SRSp
Confotec® MR520-SRSp
Confotec® MR750-SRSp
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.
Confotec® MR350-SRM
Confotec® MR520-SRM
Confotec® MR750-SRM
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.
  • High speed and high spatial resolution panoramic Rayleigh scattering imaging using a Reflected unit, XY galvano-mirrors and an automated microscope stage.
Confotec® MR350-SR
Confotec® MR520-SR
Confotec® MR750-SR
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.
  • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.
Confotec® MR350-S
Confotec® MR520-S
Confotec® MR750-S
  • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

  • Confotec® MR350-SRMSp, MR520-SRMSp, MR750-SRMSp:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.

    • High speed and high spatial resolution panoramic imaging (Raman and Rayleigh scattering) using high speed PMTs, XY galvano-mirrors and an automated microscope stage.

  • Confotec® MR350-SRSp, MR520-SRSp, MR750-SRSp:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Raman imaging (3 sec / frame, 1001 x 1001 points) using a spectral PMT and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.

  • Confotec® MR350-SRM, MR520-SRM, MR750-SRM:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.

    • High speed and high spatial resolution panoramic Rayleigh scattering imaging using a Reflected unit, XY galvano-mirrors and an automated microscope stage.

  • Confotec® MR350-SR, MR520-SR, MR750-SR:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

    • High speed 2D / 3D Rayleigh imaging (3 sec / frame, 1001 x 1001 points) using a Reflected unit and XY galvano-mirror / Z-piezo scanners.

  • Confotec® MR350-S, MR520-S, MR750-S:

    • 2D / 3D Raman mapping of a sample with full spectra detected at each point using a high speed digital CCD and XY galvano-mirror / Z-piezo scanners.

Microscope

Type, model: Upright Nikon Ni-U
Objective lenses: 100х NA-0.95, 40х NA-0.75, 20х NA-0.50, etc.
Eyepieces: 10x (optional 12.5x, 15x)
Motorized stage: for SRMSp and SRM configurations
  • travel range XY
  • position repeatability
  • metric accuracy
  • minimal step
  • X=114 mm, Y=75 mm
  • < 0.5 μm
  • 0.06 μm / 1 mm
  • 0.02 μm
Manual focusing along Z:
  • travel range
  • focusing mechanism sensitivity
  • 10 mm
  • 0.1 mm/turn
Z-scanner:
  • objective lens travel range
  • minimal step
  • repeatability
  • 80 μm
  • 50 nm
  • < 6 nm
High-resolution digital camera: with Peltier cooling (the temperature of 20 °C below the ambient temperature)
  • type
  • sensor
  • pixel size
  • ADC
  • cooling
  • digital color CCD camera
  • 4/3″, 2048 x 2048 pixels
  • 7.4 x 7.4 μm
  • 14 bit, 16 frames/sec
  • Peltier cooling
High-resolution digital camera: without cooling
  • type
  • sensor
  • pixel size
  • ADC
  • digital color CCD camera
  • 1/2″, 2048 x 1536 pixels
  • 3.2 x 3.2 μm
  • 10 bit, 12 frames/sec
Laser light input: three-position automatic turret
  • Type, model: Upright Nikon Ni-U.
  • Objective lenses: 100х NA-0.95, 40х NA-0.75, 20х NA-0.50, etc.
  • Eyepieces: 10x (optional 12.5x, 15x).

  • Motorized stage (for SRMSp and SRM configurations): travel range XY – X=114 mm, Y=75 mm; position repeatability < 0.5 μm; metric accuracy 0.06 μm / 1 mm; minimal step 0.02 μm.

  • Manual focusing along Z: travel range = 10 mm; focusing mechanism sensitivity = 0.1 mm/turn.

  • Z-scanner: objective lens travel range 80 μm; minimal step 50 nm; repeatability < 6 nm.

  • High-resolution digital camera with Peltier cooling (the temperature of 20 °C below the ambient temperature): type = digital color CCD camera; sensor = 4/3″, 2048 x 2048 pixels; pixel size = 7.4 x 7.4 μm; ADC = 14 bit, 16 frames/sec; cooling = Peltier cooling.
  • High-resolution digital camera without cooling: type = digital color CCD camera; sensor = 1/2″, 2048 x 1536 pixels; pixel size = 3.2 x 3.2 μm; ADC = 10 bit, 12 frames/sec.
  • Laser light input: three-position automatic turret.

Raman optical unit

Optimized optics for the spectral range: VIS and NIR (400-1100 nm)
Laser radiation delivery: built-in lasers – direct input;
external lasers: 633 nm – fiber input,
785 nm – direct input
Beam expander: magnification factor
2х – 10х
Edge filters unit: single position unit with two Edge filters, manual change
Confocal pinhole: optical fiber (Ø 50 μm)
Laser beam attenuator: continuously Variable Neutral Density Filter (VND),
optical density range of 0 – 3
  • Optimized optics for the spectral range: VIS and NIR (400-1100 nm).
  • Laser radiation delivery: built-in lasers – direct input; external lasers: 633 nm – fiber input, 785 nm – direct input.
  • Beam expander: magnification factor 2х – 10х.
  • Edge filters unit: single position unit with two Edge filters, manual change.
  • Confocal pinhole: optical fiber (Ø 50 μm).
  • Laser beam attenuator: continuously Variable Neutral Density Filter (VND), optical density range of 0 – 3.

Imaging monochromator-spectrograph

Microscope model Confotec® MR350 Confotec® MR520 Confotec® MR750
Monochromator-spectrograph model MS3504i MS5204i MS7504i
Optical scheme: Czerny-Turner Czerny-Turner Czerny-Turner
Ports: 1 input, 2 output 1 input, 2 output 1 input, 2 output
Focal length (output): 350 520 750
F/number (entrance): 1 / 3.8 1 / 5.4 1 / 8.9
Horizontal magnification: 1.09 1.09 1.05
Vertical magnification: 1.49 1.29 1.125
Vertical spatial resolution (astigmatism): < 15 μm < 15 μm < 15 μm
Reciprocal linear dispersion: *2.37 nm/mm *1.50 nm/mm *1.50 nm/mm
Spectral resolution: *0.07 nm **0.007 (0.25 cm-1) nm *0.02 nm
Wavelength accuracy: *±0.125 nm *±0.05 nm *±0.05 nm
Repeatability: *±0.03 nm *±0.008 nm *±0.006 nm
Grating mounting: automated 4-grating turret automated 4-grating turret automated 4-grating turret
Grating wavelength repeatability: *±0.03 nm *±0.008 nm *±0.006 nm
Grating vertical image repeatability: ±50 μm ±50 μm ±50 μm
* With 1200 l/mm grating and 15 µm slit at 546 nm.
** With Echele grating (75 l/mm) and 15 µm slit at 546 nm, order 44.

Microscope Confotec® MR350
Monochromator-spectrograph MS3504i

  • Optical scheme: Czerny-Turner

  • Ports: 1 input, 2 output

  • Focal length (output): 350

  • F/number (entrance): 1 / 3.8

  • Horizontal magnification: 1.09

  • Vertical magnification: 1.49

  • Vertical spatial resolution (astigmatism): < 15 μm

  • Reciprocal linear dispersion: *2.37 nm/mm

  • Spectral resolution: *0.07 nm

  • Wavelength accuracy: *±0.125 nm
  • Repeatability: *±0.03 nm
  • Grating mounting: automated 4-grating turret
  • Grating wavelength repeatability: *±0.03 nm
  • Grating vertical image repeatability: ±50 μm

Microscope Confotec® MR520
Monochromator-spectrograph MS5204i

  • Optical scheme: Czerny-Turner

  • Ports: 1 input, 2 output

  • Focal length (output): 520
  • F/number (entrance): 1 / 5.4

  • Horizontal magnification: 1.09

  • Vertical magnification: 1.29

  • Vertical spatial resolution (astigmatism): < 15 μm

  • Reciprocal linear dispersion: *1.50 nm/mm

  • Spectral resolution: **0.007 (0.25 cm-1) nm

  • Wavelength accuracy: *±0.05 nm
  • Repeatability: *±0.008 nm
  • Grating mounting: automated 4-grating turret
  • Grating wavelength repeatability: *±0.008 nm
  • Grating vertical image repeatability: ±50 μm

Microscope Confotec® MR750
Monochromator-spectrograph MS7504i

  • Optical scheme: Czerny-Turner

  • Ports: 1 input, 2 output

  • Focal length (output): 750
  • F/number (entrance): 1 / 8.9
  • Horizontal magnification: 1.05
  • Vertical magnification: 1.125

  • Vertical spatial resolution (astigmatism): < 15 μm

  • Reciprocal linear dispersion: *1.50 nm/mm
  • Spectral resolution: *0.02 nm
  • Wavelength accuracy: *±0.05 nm
  • Repeatability: *±0.006 nm
  • Grating mounting: automated 4-grating turret
  • Grating wavelength repeatability: *±0.006 nm
  • Grating vertical image repeatability: ±50 μm

* With 1200 l/mm grating and 15 µm slit at 546 nm.
** With Echele grating (75 l/mm) and 15 µm slit at 546 nm, order 44.

Imaging monochromator-spectrograph MS 3504i

Grating, l/mm 300 600 1200 1800
Spectral range for Raman detection, cm-1
Laser wave-length 473 nm 0-12000 0-9400
Laser wave-length 532 nm 0-9700 0-7000
Laser wave-length 633 nm 0-6700 0-4000
Laser wave-length 785 nm 0-3600 0-1000
Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1
Laser wave-length 473 nm 7000 4210 2310 1550
Laser wave-length 532 nm 5700 3400 1840 1210
Laser wave-length 633 nm 4280 2470 1300 820
Laser wave-length 785 nm 2930 1650 830 480
Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1
Laser wave-length 473 nm 12.850 6.440 3.185 2.050
Laser wave-length 532 nm 10.165 5.090 2.505 1.590
Laser wave-length 633 nm 7.190 3.595 1.745 1.070
Laser wave-length 785 nm 4.675 2.330 1.100 0.620

Spectral range for Raman detection, cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 0-12000, 0-12000, 0-12000 and 0-9400 cm-1

  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 0-9700, 0-9700, 0-9700 and 0-7000 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 0-6700, 0-6700, 0-6700 and 0-4000 cm-1

  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 0-3600, 0-3600, 0-3600 and 0-1000 cm-1

Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 7000, 4210, 2310 and 1550 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 5700, 3400, 1840 and 1210 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 4280, 2470, 1300 and 820 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 2930, 1650, 830 and 480 cm-1

Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 12.850, 6.440, 3.185 and 2.050 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 10.165, 5.090, 2.505 and 1.590 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 7.190, 3.595, 1.745 and 1.070 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 4.675, 2.330, 1.100 and 0.620 cm-1

Imaging monochromator-spectrograph MS 5204i

Grating, l/mm 300 600 1800 75/Echelle
Spectral range for Raman detection, cm-1
Laser wave-length 473 nm 0-12000 0-11000 0-12000
Laser wave-length 532 nm 0-9700 0-8700 0-9700
Laser wave-length 633 nm 0-6700 0-5800 0-6700
Laser wave-length 785 nm 0-3600 0-2700 0-3600
Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1
Laser wave-length 473 nm 6900 3500 1100 275
Laser wave-length 532 nm 5500 2760 850 255
Laser wave-length 633 nm 3910 1950 570 210
Laser wave-length 785 nm 2540 1260 330 160
Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1
Laser wave-length 473 nm 6.150 3.080 0.975 0.250
Laser wave-length 532 nm 4.865 2.430 0.755 0.230
Laser wave-length 633 nm 3.440 1.715 0.505 0.190
Laser wave-length 785 nm 2.235 1.110 0.295 0.145

Spectral range for Raman detection, cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 0-12000, 0-12000, 0-11000 and 0-12000 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 0-9700, 0-9700, 0-8700 and 0-9700 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 0-6700, 0-6700, 0-5800 and 0-6700 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 0-3600, 0-3600, 0-2700 and 0-3600 cm-1

Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 6900, 3500, 1100 and 275 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 5500, 2760, 850 and 255 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 3910, 1950, 570 and 210 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 2540, 1260, 330 and 160 cm-1

Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 6.150, 3.080, 0.975 and 0.250 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 4.865, 2.430, 0.755 and 0.230 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 3.440, 1.715, 0.505 and 0.190 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1800 and 75/Echelle l/mm: 2.235, 1.110, 0.295 and 0.145 cm-1

Imaging monochromator-spectrograph MS 7504i

Grating, l/mm 300 600 1200 1800
Spectral range for Raman detection, cm-1
Laser wave-length 473 nm 0-12000 0-9300
Laser wave-length 532 nm 0-9700 0-6900
Laser wave-length 633 nm 0-6700 0-3900
Laser wave-length 785 nm 0-3600 0-900
Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1
Laser wave-length 473 nm 3910 2130 1070 680
Laser wave-length 532 nm 3150 1690 840 520
Laser wave-length 633 nm 2280 1200 580 340
Laser wave-length 785 nm 1520 780 360 185
Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1
Laser wave-length 473 nm 3.785 1.915 0.920 0.570
Laser wave-length 532 nm 3.060 1.510 0.715 0.435
Laser wave-length 633 nm 2.160 1.060 0.490 0.285
Laser wave-length 785 nm 1.400 0.680 0.305 0.160

Spectral range for Raman detection, cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 0-12000, 0-12000, 0-12000 and 0-9300 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 0-9700, 0-9700, 0-9700 and 0-6900 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 0-6700, 0-6700, 0-6700 and 0-3900 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 0-3600, 0-3600, 0-3600 and 0-900 cm-1

Maximal spectral range detected with CCD (2048×122 pixels, 12 μm pixel size), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 3910, 2130, 1070 and 680 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 3150, 1690, 840 and 520 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 2280, 1200, 580 and 340 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 1520, 780, 360 and 185 cm-1

Spectral resolution with CCD (12 μm pixel size, 14 μm entrance slit), cm-1

  • Laser wave-length 473 nm, grating 300, 600, 1200 and 1800 l/mm: 3.785, 1.915, 0.920 and 0.570 cm-1
  • Laser wave-length 532 nm, grating 300, 600, 1200 and 1800 l/mm: 3.060, 1.510, 0.715 and 0.435 cm-1
  • Laser wave-length 633 nm, grating 300, 600, 1200 and 1800 l/mm: 2.160, 1.060, 0.490 and 0.285 cm-1
  • Laser wave-length 785 nm, grating 300, 600, 1200 and 1800 l/mm: 1.400, 0.680, 0.305 and 0.160 cm-1

Digital spectral CCD camera

Sensor type: back-thinned CCD, 2048 x 122 pixels
Pixel size: 12 x 12 μm
Active area size: 24.576 x 1.464 mm (W x H)
Wavelength range: from 200 to 1100 nm
Cooling with temperature stabilization: two stage Peltier cooling, up to -45 °С (water cooling)
ADC: 16 bit
Sensitivity: 1 photon / 1 count ADC (at 650 nm)
Dynamic range: not less than 10 000
Synchronization: internal, external
Interface: Ethernet
  • Sensor type: back-thinned CCD, 2048 х 122 pixels.
  • Pixel size: 12 x 12 μm.
  • Active area size: 24.576 x 1.464 mm (W x H).

  • Wavelength range: from 200 to 1100 nm.

  • Cooling with temperature stabilization: two stage Peltier cooling, up to -45 °С (water cooling).
  • ADC: 16 bit.

  • Sensitivity: 1 photon / 1 count ADC (at 650 nm).

  • Dynamic range: not less than 10 000.
  • Synchronization: internal, external.
  • Interface: Ethernet.

PMT for spectral measurements

A Hamamatsu PMT unit (model H7844) with the built-in one-stage air cooled Peltier element is used for Raman spectra detection:

Spectral range: 185 – 900 nm
Photocathode light sensitivity: 450 μА/lm
Anode light sensitivity: > 5000 А/lm
Anode dark current
(in 3 minutes after start cooling):		 < 0,05 nA
Max. cooling temperature (ΔТ): 20 ºС
  • Spectral range: 185 — 900 nm
  • Photocathode light sensitivity: 450 μА/lm
  • Anode light sensitivity: > 5000А/lm
  • Anode dark current (in 3 minutes after start cooling): less than 0,05 nA
  • Max. cooling temperature (ΔТ): 20 ºС

Fast scanning unit X, Y

Scanners: galvanometer scanners with X, Y mirrors
Scanning modes: raster high-speed and start-stop
Positioning accuracy: < 30 nm
Scanning area: 100 μm х 100 μm (with 100Х objective lens)
Scanning speed: 3 s/frame 1001 х 1001 pixels
  • Scanners: galvanometer scanners with X, Y mirrors.
  • Scanning modes: raster high-speed and start-stop.

  • Positioning accuracy: < 30 nm.

  • Scanning area: 100 μm х 100 μm (with 100Х objective lens).
  • Scanning speed: 3 s/frame 1001 х 1001 pixels.

Confocal laser microscope unit

Pre-pinhole lens positioner: three-axis (X, Y, Z), manual control
Confocal pinhole: unregulated, Ø 30 μm
Detector: Hamamatsu Photosensor module H6780-01
  • Pre-pinhole lens positioner: three-axis (X, Y, Z), manual control.
  • Confocal pinhole: unregulated, Ø 30 μm.
  • Detector: Hamamatsu Photosensor module H6780-01.

Lasers: built-in and external

532 nm built-in laser

Type: ОЕМ Diode-pumped solid-state laser (DPSS)
Wavelength: 532 nm
Output power: 22±2 mW
Output beam diameter: 1.1 ± 0.1 mm
Beam divergence: < 0,6±0.1 mrad
Spectral line width: 0.00001 nm
Coherence length: > 50 m
Transverse mode: TEMoo, M2 < 1.2 (1.05 typ.)
Polarization: > 100:1, horizontal
Long term power stability: < 2% / 8 h.
Warm-up time: < 10 minutes
  • Type: ОЕМ Diode-pumped solid-state laser (DPSS)
  • Wavelength: 532 nm
  • Output power: 22±2 mW
  • Output beam diameter: 1.1 ± 0.1 mm

  • Beam divergence: < 0,6±0.1 mrad

  • Spectral line width: 0.00001 nm
  • Coherence length: > 50 m

  • Transverse mode: TEMoo, M2 < 1.2 (1.05 typ.)

  • Polarization: > 100:1, horizontal

  • Long term power stability: < 2% / 8 h.

  • Warm-up time: < 10 minutes

473 nm built-in laser

Type: ОЕМ Diode-pumped solid-state laser (DPSS)
Wavelength: 473 nm
Output power: 50 mW
Output beam diameter: 0.7 ± 0.05 mm
Beam divergence: < 1.2 mrad
Spectral bandwidth: < 30 MHz
Coherence length: > 10 m
Transverse mode: TEMoo, M2 < 1.1
Polarization: > 100:1, vertical
Long term power stability: < 3% / 8 h.
Warm-up time: < 10 minutes

  • Type: ОЕМ Diode-pumped solid-state laser (DPSS)

  • Wavelength: 473 nm
  • Output power: 50 mW
  • Output beam diameter: 0.7 ± 0.05 mm

  • Beam divergence: < 1.2 mrad

  • Spectral bandwidth: < 30 MHz

  • Coherence length: > 10 m

  • Transverse mode: TEMoo, M2 < 1.1

  • Polarization: > 100:1, vertical

  • Long term power stability: < 3% / 8 h.

  • Warm-up time: < 10 minutes

633 nm external laser

Type: Helium-neon laser
Wavelength: 632,8 nm
Output power: > 10 mW
Output beam diameter: 0.65 mm
Beam divergence: < 1.24 mrad
Spectral bandwidth: 341 MHz
Mode composition: > 90 % TEMoo
Polarization: 500:1
Long term power stability: < 5% / 8 h.
Beam point stability: < 0.03 mrad/°C
Warm-up time: < 15 minutes
  • Type: Helium-neon laser
  • Wavelength: 632,8 nm

  • Output power: > 10 mW

  • Output beam diameter: 0.65 mm
  • Beam divergence: < 1.24 mrad
  • Spectral bandwidth: 341 MHz

  • Mode composition: > 90 % TEMoo

  • Polarization: 500:1
  • Long term power stability: < 5% / 8 h.

  • Beam point stability: < 0.03 mrad/°C

  • Warm-up time: < 15 minutes

785 nm external laser

Type: ОЕМ Diode-pumped solid-state laser (DPSS)
Wavelength: 785 ± 0.2 nm
Output power: 100 mW
Output beam diameter: 1.5 mm
Beam divergence: < 1.0 mrad
Spectral bandwidth: < 1 MHz
Beam quality: M2 < 1.65
Polarization: vertical ±5°
Long term power stability: < 1% / 8 h.
Warm-up time: < 10 minutes
  • Type: ОЕМ Diode-pumped solid-state laser (DPSS)
  • Wavelength: 785 ± 0.2 nm
  • Output power: 100 mW
  • Output beam diameter: 1.5 mm

  • Beam divergence: < 1.0 mrad

  • Spectral bandwidth: < 1 MHz

  • Beam quality: M2 < 1.65

  • Polarization: vertical ±5°
  • Long term power stability: < 1% / 8 h.

  • Warm-up time: < 10 minutes

Microscope models

Microscope configuration models Confotec® MR350/520/750-SRMSp Confotec® MR350/520/750-SRM Confotec® MR350/520/750-SRSp Confotec® MR350/520/750-SR Confotec® MR350/520/750-S
Mapping 3 D (X Y Z) 3 D (X Y Z) 3 D (X Y Z) 3 D (X Y Z) 3 D (X Y Z)
Scanning: XY galvano + motorized stage, Z piezo XY galvano, Z piezo XY galvano, Z piezo XY galvano, Z piezo
Fast scanning for confocal Raman imaging: Yes – Yes – –
Fast scanning for confocal laser imaging: Yes Yes Yes Yes –
Data: 2D, 3D 2D, 3D 2D, 3D 2D, 3D 2D, 3D
Microscope Upright Upright Upright Upright Upright
Color video CCD: Yes Yes Yes Yes Yes
Raman optical unit
Location: integrated with an optical microscope
Optical alignment: no need
Rayleigh rejection filters: two edge filters, manually replaced
Imaging monochromator-spectrograph
Focal length: 350/520/750 mm
Exit ports: 2 1 2 1 1
Exit port selection: automated exit mirror
Number of gratings: 4 4 4 4 4
Grating mounting: automated 4-grating turret
Light input: one-channel optical fiber
Digital spectral CCD camera
Number of pixels: 2048 x 122
Pixel size: 12 x 12 µm
Cooling with temperature stabilization: two-stage Peltier cooling with temperature stabilization, up to -45°С (water cooling)
Excitation lasers (1 built-in and 1 external)
Wavelength of built-in laser: 473 nm or 532 nm
Light input of built-in laser: direct
Wavelength of external laser: 633 nm or 785 nm
Light input of external laser: 633 nm – fiber, 785 nm – direct
Laser beam diameter control: zoom beam expander, manual
Laser power control: continuous VND (OD0-3), motorized
Confocal pinhole
Confocal Raman imaging: fiber Ø 50 μм
Confocal laser imaging: fixed Ø 30 μм fixed Ø 30 μм –
Reflected Unit: Yes Yes Yes Yes –
Built-in calibration lamp: Yes
Spectral PMT: Yes – Yes – –
Motorized microscope stage: Yes Yes – – –

Confotec® MR350/520/750-SRMSp

  • Mapping 3D (X Y Z): scanning = XY galvano + motorized stage, Z piezo; fast scanning for confocal Raman imaging; fast scanning for confocal laser imaging; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 2; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.
  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; confocal laser imaging = fixed Ø 30 μm; Reflected Unit; built-in calibration lamp; spectral PMT; motorized microscope stage.

Confotec® MR350/520/750-SRM

  • Mapping 3D (X Y Z): scanning = XY galvano + motorized stage, Z piezo; fast scanning for confocal laser imaging; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 1; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.
  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; confocal laser imaging = fixed Ø 30 μm; Reflected Unit; built-in calibration lamp; motorized microscope stage.

Confotec® MR350/520/750-SRSp

  • Mapping 3D (X Y Z): scanning = XY galvano, Z piezo; fast scanning for confocal Raman imaging; fast scanning for confocal laser imaging; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.

  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 2; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.

  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; confocal laser imaging = fixed Ø 30 μm; Reflected Unit; built-in calibration lamp; spectral PMT.

Confotec® MR350/520/750-SR

  • Mapping 3D (X Y Z): scanning = XY galvano, Z piezo; fast scanning for confocal laser imaging; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 1; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.
  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; confocal laser imaging = fixed Ø 30 μm; Reflected Unit; built-in calibration lamp.

Confotec® MR350/520/750-S

  • Mapping 3D (X Y Z): scanning = XY galvano, Z piezo; data = 2D and 3D.
  • Microscope: upright; color video CCD.
  • Raman optical unit: location = integrated with an optical microscope; no need in optical alignment; Rayleigh rejection filters = two edge filters, manually replaced.
  • Imaging monochromator-spectrograph: focal length = 350/520/750 mm; exit ports = 1; exit port selection = automated exit mirror; number of gratings = 4; grating mounting = automated 4-grating turret; light input = one-channel optical fiber.
  • Digital spectral CCD camera: number of pixels 2048 x 122; pixel size 12 x 12 µm; cooling with temperature stabilization = two stage Peltier cooling, up to -45 °С (water cooling).
  • Excitation lasers (1 built-in and 1 external): wavelength of built-in laser 473 nm or 532 nm; light input of built-in laser = direct; wavelength of external laser 633 nm or 785 nm; light input of external laser = 633 nm – fiber, 785 nm — direct; laser beam diameter control = manual zoom beam expander; laser power control = continuous VND (OD0-3), motorized.
  • Confocal pinhole: confocal Raman imaging = fiber Ø 50 μm; built-in calibration lamp.